Characteristics of compacted plasmid DNA by high pressurization
نویسندگان
چکیده
منابع مشابه
Ultrafiltration characteristics of plasmid DNA
Plasmids are extra-chromosomal circular double-stranded DNA molecules that can be used to develop recombinant cell lines and to deliver therapeutic genetic material. In gene therapy applications, the in-vivo insertion of the genetic vector results in the production of a desired therapeutic bio-molecule. Gene therapy is of interest in the treatment of a wide range of medical conditions including...
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We describe a simple calcium phosphate transfection protocol and neo marker vectors that achieve highly efficient transformation of mammalian cells. In this protocol, the calcium phosphate-DNA complex is formed gradually in the medium during incubation with cells and precipitates on the cells. The crucial factors for obtaining efficient transformation are the pH (6.95) of the buffer used for th...
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Streptococcus lactis IL1403 protoplasts were transformed by plasmid pIL204 (5.5 kilobases), which conferred erythromycin resistance with an average efficiency of 5 X 10(6) transformants per microgram of supercoiled DNA. The procedure used and transformation efficiencies obtained were close to those described for Bacillus subtilis (G. Chang and S. N. Cohen, Mol. Gen. Genet. 168:111-115, 1979).
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ژورنال
عنوان ژورنال: Nucleic Acids Symposium Series
سال: 2007
ISSN: 0261-3166,1746-8272
DOI: 10.1093/nass/nrm172